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https://www.fishlore.com/aquariumfishforum/threads/q-a-with-tetra-about-tetra-safestart.58116/ I got the idea that Prime is bad for BBs from the link that I posted above. OK , now the condensed long version of how Sebastian's tank got cycled. Prior to getting Sebastian, I had a betta named Leo in a ten gallon . I used BBs in a bottle to following the exact instructions of the link that I linked. Leo's tank has been fully cycled for a month. When I got Sebastian, I got Sebastian a twenty gallon tank . To cycle Sebastian's tank, I used all the bio media in Leo's tank to instantaneously cycle Sebastian's tank. I'm cycling Leo's tank again with BBs in a bottle. OK now to my question. I intend to do a 50% water change on Sebastian tank. I intend to add Prime at the same time as I do the water change. Do I have to worry that Prime will kill the BBs in the HOB and in the tank ? A picture of Leo below.